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Next generation sequencing and quantitative polymerase chain reaction technologies are now widely available, and research incorporating these methods is growing exponentially.
To detect and identify pathogens, among widely used approaches are the traditional sample cultivation protocols, enzyme-linked immunosorbent assay, immunomagnetic separation, surface plasmon resonance detection, and nucleic acid-based polymerase chain reaction technologies [2 5].
Nevertheless, the results obtained with polymerase chain reaction technologies may be skewed by methodological contaminants.
Western blot and reverse-transcription polymerase chain reaction technologies were used to detect the expression of p21, CDK2 and cyclinE in RPE cells and rabbit retinal tissues.
After the feeding trials, we isolated RNAs from both brain and liver tissues and analyzed the expression profiles of 567 known mouse miRNAs using microarray and quantitative real-time polymerase chain reaction technologies.
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A few years ago the historian Edward Tenner argued in "Why Things Bite Back: Technology and the Revenge of Unintended Consequences" that for every technological action, there is an equal, opposite and unpredicted reaction: technology's revenge.
The critics agree that contamination can be a serious issue when using polymerase chain reaction technology.
The new papers in Retrovirology reported that contamination of tissue samples or other laboratory items with mouse DNA or viral genetic material could lead to false positive results for XMRV, and by extension other MLV-related viruses, specifically when using polymerase chain reaction technology.
Using the latest polymerase chain reaction technology and techniques, his team has sequenced millions of base pairs of nuclear DNA from mammoths and other extinct species.
PbSe/CdSe core/shell nanocrystals with quantum yield of 70% were obtained by the "successive ion layer adsorption and reaction" technology in solution.
With the precisely controlled reaction temperature (285 °C) and residence time (10 s) by the recently introduced microfluidic reaction technology, green luminescent CdSe NCs (λ = 522 nm) exhibiting narrow FWHM of PL (30 nm) was reproducibly obtained.
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