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A functional gene-based approach employing terminal restriction fragment length polymorphism and quantitative polymerase chain reaction targeting genes of sulfate-reducing (dsrA), cellulose-degrading (cel5, cel48), fermentative (hydA), and methanogenic (mcrA) microbes was applied.
Three separate robust PCR reactions were formulated, 2 for selective amplification of portions of only the true gene exons 2 and 9, with a third reaction targeting exon 10, wherein both the true and pseudogene were coamplified.
Only a single fluorescent colony was identified on a plate containing 33 colonies and deriving from a mutagenesis reaction targeting 5 residues.
A PCR amplification reaction targeting the deletion breakpoint in normal, heterozygous and homozygous mutant individuals with the Filipino deletion was modified to detect simultaneously the normal and mutant alleles using three PCR primers 45F: 5' TCA GAA GCA GAG CTA CTC AG 3'; 45R1- 5' GTC TAT GCA GGT GTG TAG ACA 3' and 45R2: 5' CAT TTA GCT CCC ACA CTC CT 3'.
We tested a single early-morning sputum specimen for Mycobacterium tuberculosis DNA using two nucleic acid amplification tests: a novel in-house polymerase chain reaction targeting the mycobacterial secA1 gene, and the commercial Amplified® Mycobacterium tuberculosis Direct (MTD) test Gen-Probe Incc, San Diego, CA).
Strains were serotyped by the Quellung reaction targeting 93 serotypes [ 5].
Similar(47)
Since strand cleavage by the Tyr-343 nucleophile is accompanied by inversion of configuration at the phosphate center, the hydrolysis reaction targets the MeP-tyrosyl intermediate whose chirality is opposite to that of the starting SP MeP-half-site.
We believe that vitamin D, in some fashion, might inhibit the autoimmune reaction targeted toward cells of the pancreas [ 13].
The metabolic engineering targets identified using the metabolite-centric technique can be equivalent to the reaction targets obtained by the gene deletion-based approach.
The reparatory nature of the lesions suggests that the inflammation is the result of direct tissue damage rather than an immune reaction targeted toward the parasitic antigens.
A long PCR amplification generated a fragment of ~12,000 base pairs (bp) and a second reaction targeted a fragment of ~4,500 bp.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com