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The catalysts stability and reusability were confirmed in eight consecutively reaction runs.
The robustness and flexibility of the software sensor are examined on simulated reaction runs.
Reaction runs were carried out under stoichiometrically excess steam conditions between 773 and 823 K.
Adsorption and reaction runs were performed using aqueous solutions of a model azo dye Orange G.
Tr6 immobilized on MNPs gave a biocatalytic productivity of 84 mg 3′-sialyllactose/mg Tr6 after seven consecutive reaction runs.
The Cu Zr interaction results also in easy reduction and reoxidation of copper species in the reaction runs.
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The reaction ran for 12 h.
However the efficacy of catalyst dilution for preventing reaction run-away has not been tested experimentally.
The pH of the reaction mixture was found to be constant during the reaction run.
In each reaction run, the mixture was raised to 50 °C under stirring (1000 rpm).
A change to concentration or temperature may make a reaction run backwards.
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