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[Photo credits from left to right: Full Moon necklace in gold; porcelain Reaction cup; Undergrowth earrings; Radiolaria brooch; porcelain Reaction plate; Cell Cycle rings].
The reaction plate was held at 10°C until loaded on 3730xl DNA analyzer.
The reaction plate was pre-read at this point to measure any auto-fluorescence.
Real-time PCR was performed using PCR Universal Master Mix Applied Biosystemss) in a MicroAmp 96-well reaction plate.
All reactions were performed using an Applied Biosystems 7900HT real-time PCR instrument equipped with a 384 well reaction plate.
Samples were added to the reaction plate and signal amplification by PCR was carried out using Sybr-Green '1 step qRT-PCR kit' (Invitrogen).
Master-mix containing the P450 baculosomes reagent, regeneration system, and reaction buffer were added to the designated well of the reaction plate.
The amplifications were performed using a MicroAmp optical 96-well reaction plate (PE Applied Biosystems) on an ABI 7500 Real-Time PCR System (Applied Biosystems).
QRT-PCR reactions were performed with the Fast SYBR® green PCR master mix in a MicroAmp optical 96-well reaction plate according to the manufacturer's instructions (Applied Biosystems).
The reaction plate was tested by ELISA for presence of the viral nucleoprotein using mouse specific monoclonal antibody (Chemicon, Temecula, CA).
Each reaction was run in triplicate, and each sample was also run against the endogenous control on the same reaction plate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com