However, the scale of these effects depended on the kinetics of the individual thioredoxin oxidation reactions with some reactions more sensitive to changes in the thioredoxin cycle and others, such as the Tpx-dependent reduction of hydrogen peroxide, less sensitive to these changes.
The predictions suggest that the CH4 conversion is linearly proportional to the reaction temperature at the early stage; the reaction is more sensitive to the reaction temperature at the steady state.
The polymerase chain reaction was more sensitive than culture and stained smear examination in the diagnosis of cutaneous leishmaniasis, detecting 80% of cases compared to 42% and 72%, respectively.
19 The polymerase chain reaction is more sensitive than microscopic examination and, therefore, it is often the primary test in referral hospitals and research centers, with quantitative polymerase chain reaction permitting accurate diagnosis using venous blood samples instead of bone marrow aspirates.
We adapted the original protocol by Perelle et al. [ 26] for use in a duplex reaction but eventually found that, despite the duplex assay based on these primers and probe guaranteed high specificity, single reactions were more sensitive than duplex.
This indicates that the reverse reaction rate is more sensitive to temperature than the forward reaction rate.
Molecular methods such as real-time polymerase chain reaction (PCR) are more sensitive, detecting <10 genome equivalents per PCR, but PCR can only identify short stretches of poxvirus DNA (1, 7 ).
DOI: http://dx.doi.org/10.7554/eLife.02109.004 As we observed high and stable performance levels at different dilutions, we calculated the reaction times, a more sensitive parameter to monitor discrimination behavior (Abraham et al., 2004, 2010, 2012).
Reverse transcriptase polymerase chain reaction is even more sensitive, but it is difficult to find a RNA sequence that is specific to tumours; it may be necessary to use a panel of markers, particularly as the morphology of the positive cells cannot be assessed (Liefers et al, 1999).
All samples with undetectable BCR-ABL are also examined using digital polymerase chain reaction, which is a more sensitive nanofluidic polymerase chain reaction system.
