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Whereas the multiple-turnover rate monitors the entire reaction, including product release, the single-turnover reaction monitors only steps up to and including N-glycosidic bond hydrolysis, which is irreversible under these conditions (Scheme 1).
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Anderson, L. & Hunter, C. L. Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins.
Two common acquisition strategies were utilized: DDA and selected reaction monitoring (SRM).
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Peptide selection and multiple reaction monitoring design are discussed.
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Selected reaction monitoring (SRM), was completed on a Thermo Vantage for all bacterial dilution samples in Table 1.
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