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To achieve dextran succinnylation, 1-hydroxy benzotriazole (0.675 g, 5.0 mmol) and disuccinimidyl suberate (1.841 g, 5.0 mmol) were dissolved in DMSO (60 mL) and heated to 80°C for 5 min. After cooling, 1-amino-dextran (10 kDa) (5.0 g, 0.5 mmol) was added and the reaction maintained at 20°C for 24 h.
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In response to a question, Fay also stated that he had not been affected by the "scary" fan reaction, maintaining that "a universe in which fictional characters aren't 'allowed' to die is ridiculous and limiting".
Sixty ng of PCR product was used for each cloning reaction, maintaining a molar ratio insert : vector of 3 : 1.
Second, from the remaining removable reactions, the next deletion candidate is identified as the reaction that has the smallest overall flux range F i. We assume that removal of this reaction maintains a high degree of flux variability in the network (other criteria for selecting the next deletion candidate could be used as well).
How are the nonactive reactions maintained in the metabolic network?
Limited spontaneous reactions maintain constant apparent activation energy for the oxidation until the chain reactions are generated; the apparent activation energy then increases.
The study suggested that GRXs have a broad capacity to reduce oxidized disulfides and to provide electrons to many enzymatic reduction reactions maintaining redox homeostasis.
Species connected by a chain of sequential reversible reactions maintain a correlation significantly different from zero during all the observation time.
The ultrashort activated ET-1 and NOS following SAH lead to transient vasoconstriction and a cascade of followed neuroinflammatory reactions maintains the intensity of vasoconstriction.
Because of the characteristics of a sub-critical reaction process, the fission chain reaction is maintained by additional neutrons generated by protons in spallation target.
Since the reaction was maintained at subcritical condition under nitrogen environment without the presence of any catalyst, the oxidation or secondary reaction of the biodiesel product did not occur.
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