Sentence examples for reaction lanes from inspiring English sources
Furthermore, this hydrolysis product and the synthetic 24-mer served as primers in a sequenase primer extension reaction (lanes 5, 7, Figure 6B).
Recombinant SLAC1-NT phosphorylation by CPK21 (lane 1) is inhibited if the protein phosphatases ABI1 and PP2CA are added before starting the reaction (lanes 2 3).
As illustrated in Figure 3B, bands of the expected sizes, and the expected sizes only, were easily detected in all reaction lanes, demonstrating tolerance of the assay for a wide range of template concentrations.
Vam7p (0.5 µM) was added where indicated, either during the fusion reaction (lanes 2 and 5) or after the reaction was terminated by detergent addition (indicated by an asterisk, lanes 3 and 6).
The cleavage efficiency was greatly improved when the DTT concentration was increased to 100 mM), and most of the fusion tag was released after a 24 h reaction, lanes F1 and F2), producing a clear band corresponding to the molecular weight of the OG2, lanes P1 and P2).
However, while one molar equivalent of (dT 24 reduced the bound Rpa by a factor of ∼4, the remaining Rpa was clearly above the background level of the GST-only reaction (lanes 4 and 5), suggesting that only one of the two Dna2-Rpa interactions is mutually exclusive with Rpa-ssDNA interactions.
This signal was absent when no exogenous RNA was added to the reaction (lane 2).
For quantitation of strand displacement the percent of displaced products (full length and shortened) were calculated as a function of the total radioactivity in the reaction lane [37].
It co-migrated with the appropriate band of the sequencing ladder and slightly below the synthetic 24-mer (lanes 1 3, Figure 6B), and failed to serve as a primer in the chain extension reaction (lane 5, Figure 6B).
A +15 nucleotide toeprint band was detected in the presence of cycloheximide alone (lane 6) and its intensity was enhanced when sparsomycin was also added to the reaction (lane 7).
The lower band was inferred to contain a 3'-methylphosphate end and the upper one a 3'-OH end, based on their behavior in the sequenase reaction (lane 9, Figure 6C).
