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Direct sequencing of the PCR products was performed with an ABI BigDye terminator v3.1 sequencing Ready Reaction kit.
Immunoreactivity was detected with the ECL-chemoluminescence reaction kit (Amersham).
Immunoreactivity was detected with the ECL-chemiluminescence reaction kit (Amersham, Little Chalfont, UK).
From each product both strands of eight clones were sequenced, using the ABI prismBigDye Terminator Cycle Sequencing Ready Reaction kit and analysed using 3730 DNA analyser.
Sequencing was performed by polymerase chain reaction-based sequencing (ABI Prism big dye terminator cycle sequencing ready reaction kit, Applied Biosystems).
Reactions were performed using BigDye-Terminator™ v3.1 Cycle Sequencing Reaction Kit on a 3730 xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) following the manufacturer's instructions.
Forty-eight hours after infection, cells were washed and assayed for luciferase activity with the Luciferase Reaction Kit (Promega) according to the manufacturer's instructions.
DNA sequencing reactions were performed using the 'DNA dRhodamine Terminator Cycle Sequencing Ready reaction' kit (Applied Biosystems) and an ABI3730xl sequencer (Applied Biosystems) according to the manufacturer's instructions.
Automated fluorescence sequencing was carried out with a BigDye Terminator Ready Reaction Kit (Applied Biosystems) on a 3130XL Genetic Analyser (Applied Biosystems) according to manufacturer's protocols.
They were then sequenced in both directions with specific primers and a D-rhodamine Terminator cycle sequencing ready reaction kit (Applied Biosystems).
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AmpliTaq Taq polymerase and BigDye-Terminator-Cycle-Sequencing-Ready-Reaction kit were supplied by Perkin Elmer (San Jose, CA, USA).
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