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The cDNA reaction for each sample contained 1 μg of total RNA.
The real time PCR reaction for each sample was run in duplicate.
A correlation between sensitivity and the catalytic efficiency of the enzyme reaction for each phenolic substrate was found.
The detection limits were found between 5.0 and 0.5 IFU/CFU per PCR reaction for each of the bacteria.
The sensitivity of this assay was determined to be less than 85 CFU per reaction for each specimen type analyzed.
Three biological replicates were used for each sample and reaction for each test sample was repeated three times.
The sensitivity of triple RT-PCR was found to be 102 copies per reaction for each of NP, H9 and N2 gene.
The heats of reaction for each of these steps are 550, 270 and 540 J/g, respectively, measured per gram of Li0.5CoO2.
The effectiveness of the surface photogeneration reaction for each polymeric structure was determined in terms of half-life of the trap decay.
The third reaction for each cell line contained all three necessary components for the ubiquitylation reaction.
This highly sensitive nested quantitative PCR assay allows the detection of one copy per PCR reaction for each DNA circle.
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CEO of Professional Science Editing for Scientists @ prosciediting.com