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It is a chain reaction failure of building members to an extent disproportionate to the original localized damage (DoD 2013).
In our study, percent reaction failure explained most of the variance in the differences between observed and expected heterozygosities (Table 1).
To rule out possibilities of PCR reaction failure, single and multiplex PCRs were conducted thrice followed by Southern blot hybridization using [32Pα-dCTP] labeled PCR products of the corresponding STSs of the unexposed males (not shown).
The null alleles were confirmed after several repetitions with different amplification conditions to ensure that no reaction failure existed.
For non-amplified cDNA, high reaction failure rates with BioMark arrays were observed below 103 copies/ng, which equates to 2 copies per 9 nL reaction.
In this study, EGFR TMDA showed to be a robust method for mutational analysis since no reaction failure was observed in the analyzed samples.
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In the latter case, the distinction between genotyping reaction failures and monomorphic SNPs is not obvious.
Nevertheless, cloning and sequencing helped us to verify questionable or unclear SNaPshot™ results by permitting the identification and differentiation of cryptic contaminations, PCR artefacts and SNaPshot™ reaction failures.
Reaction failures could happen in metabolic systems, and different reaction failures have different impacts on the robustness of the metabolic function.
Robustness characters the ability of metabolic systems to behavior normally under reaction failures.
In what follows, we focus on the impacts of different reaction failures on the robustness (whether the network is controllable with the same MDMS under reaction failures) of the controllability in the HLMN.
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