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After complete addition of APiB-POSS, the reaction content was stirred for 20 h at ambient temperature.
As described in Methods, we represented reaction content of the models by EC numbers.
It shows that metabolic networks are highly diverse in their reaction content.
Recently, an approach based on metabolic pathway reaction content was proposed [ 15].
This initial model was equivalent in reaction content to commonly used isotopomer models for E. coli [ 25, 26].
However, little is known about the variation in reaction content of the different possible metabolic networks having the same phenotype.
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The reaction contents were stirred vigorously for at least 4 h to ensure the completion of the reaction.
The reaction contents were cooled to room temperature, followed by the addition of excess methanol to flocculate the nanoparticles.
To the reaction contents, 1% of sebacoyl chloride was added in order to generate carbonyl chloride terminal ends.
The reaction contents were then cooled, mixed with a water:cyclohexane mixture and the organic layer was separated, dried over sodium sulfate and the cyclohexane was removed on a rotary evaporator.
The PCR reaction contents of the second round reaction were the same as the first round, but the N. fuckeliana PCR primers were used and 1 50 dilution of the first-round PCR products were used as the DNA template for the second round PCR reaction.
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