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The 1,2,3-triazole linkage formed in the cycloaddition reaction (click reaction) is thermodynamically and hydrolytically stable.
Then, reaction of the later compounds with propargyl bromide followed by azide alkyne cycloaddition reaction (click reaction) led to the formation of the title compounds in good yields.
The synthesis of novel tetrameric and hexameric mannoside clusters bearing 1,2,3-trizole linkages via Cu(I -catalyzed azI -catalyzedycloazide alkynecycloaddition chemistreactiondesclickd.
It was found that Cu(I -catalyzed alkyne–azI -catalyzedition realkyne azidek cycloadditiontween the alkyne-labeled glycan and the azide-tagged HSA led to an efficient foreactionof the glyclickjugates.
A series of imidazo[2,1-b]thiazole linked triazole conjugates were synthesized by using Huisgen 1,3-dipolar cyclo-addition reaction (click chemistry approach) and evaluated for their antiproliferative activity against some human cancer cell lines like, HeLa (cervical), DU-145 (prostate), A549 (lung), MCF-7 (breast) and HepG2 (liver).
Furthermore, we demonstrated the convenience of the 2′- O- 2-azidoethyl) RNA label in a typical azide–alkyne 1,3-dipO- 2-azidoethyliO- 2-azidoethylick chemistRNA.
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EdU were detected by fluorescent-azide coupling reaction (Click-iT, Invitrogen).
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The cells were washed twice with 3% BSA in PBS and then incubated with the Click-iT reaction cocktail (Click-iT reaction buffer, CuSO4, Alexa Fluor 488 Azide, and reaction buffer additive) for 30 min while being protected from light.
In this context, synthetic chemist invented simple easy reaction called click chemistry with high substrate tolerance reaction irrespective of the environment to address multidisciplinary drug development.
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