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MR buffer, metathesase reaction buffer; cat., catalyst.
The mixture was then assessed with 1 × PK reaction buffer.
Elution from the beads is achieved in an acidic reaction buffer before incubation with the cofactor.
The reaction buffer for CMTr2 differed in pH (7.4) and KCl concentration (50 mM).
The reaction buffer for CMTr2 differed with regard to pH (7.4) and KCl concentration (50 mM).
To each well containing iminobiotin beads, add the metathesase reaction buffer (97 μl).
Add 2× concentrated reaction buffer (100 μl) into the preincubated cell lysates or the CFEs.
IVT reaction buffer is extremely high in salt, so make sure the salt is fully dissolved before using the buffer.
After 30-min incubation, the samples were filtered through a nitrocellulose membrane and washed with a reaction buffer.
The reaction buffer for CMTr2 differed with regard to the pH (7.4) and KCl concentration (50 mM).
Anti-species secondary antibody diluted in Click-iT® reaction buffer was included in the EdU detection step.
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