The chromatin immunoprecipitation quantitative polymerase chain reaction assays (ChIP-qPCRs) were performed on chromatin extracted from neural cell cultures.
SLPI-specific primers were designed for use in quantitative-competitive reverse-transcription polymerase chain reaction assays to detect and quantify SLPI mRNA expression levels.
In the future, polymerase chain reaction assays, microarray testing, genomic analysis, and metabolic profiling will be accomplished in a rapid, portable, sensitive, and cost-efficient manner.
The pedigree mismatch, mixed lymphocyte reaction assays (MLRs) and allogeneic skin graft experiments were performed to confirm unrelated ASC donors and recipients.
By chromatin immunoprecipitation-polymerase chain reaction and reverse transcription-polymerase chain reaction assays, we showed that SHR regulates cytokinin homeostasis by directly controlling the transcription of cytokinin oxidase 3, a cytokinin catabolism enzyme preferentially expressed in the stele.
In addition, 12 t(14 18 -positive 14 18 -positiveby polymerase cases reaction assays on adetectedl samples were added to the fluorescence in situ hybyidization-polymeraseases for subsequent analysis.
The replicon made copious copies of itself, which the researchers showed both by polymerase chain reaction assays and by analyzing viral proteins.
Investigations for viral CNS infections (e.g. polymerase chain reaction assays for human herpesviruses or JC virus) were not available at the time of the study.
With a view to address this unmet need we developed two novel colour reaction assays that can be used in the field to check the quality of ARTs.
We screened these specimens using five multiplex real time Reverse Transcription and/or Polymerase Chain Reaction assays for detection of 14 respiratory viruses.
Amplification reaction assays contained SYBR Green PCR mastermix (Roche) and primers at the optimal concentrations.
