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The source terms for the presumed one-step chemical reaction are extracted from the rate of fuel consumption in laminar premixed hydrocarbon flames, computed using a detailed chemical kinetic mechanism.
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The products of this reaction were extracted from a 1% low-melt Seaplaque agarose gel with QIAEX II gel extraction Kit (QIAGEN) and cloned into pJAZZ-OC vector using the BigEasy v2.0 Linear Cloning Kit (Lucigen Co. Middleton, WI).
Rate constants of the reaction were extracted.
Interfacial and kinetic parameters for the cathodic reaction were extracted from cathodic transients.
Total RNA for the RT reaction was extracted using a RNeasy mini Kit (Qiagen, Hilden, Germany) from C. purpurea cells cultured in C medium37 for 1 month at 28 °C.
The reaction was extracted with ethyl acetate (50 ml), and the organic layer was washed with brine, dried (Na2SO4) and concentrated under reduced pressure.
The source from which the reaction was extracted was examined to determine whether the chemical name identified in the heading text by OSCAR3 and from which the product CML molecule was generated agreed with that stated in the heading text.
The properties of this model serpentine mineral, the other phases, and this dehydration reaction were extracted from the literature (including Robinson et al. (1983)) and are given in Additional file 1: Table S1.
For RNA gel analyses, after the polymerization assays, total RNA from each reaction was extracted by the lithium chloride (LiCl) method [33].
The reaction was extracted with ethyl acetate and cold water.
The total reaction was extracted on 1 mL chloroform.
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