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However, the kinetics of the reaction are difficult to control and appreciable amounts of the fully hydrolysed and therefore unreactive photosensitiser are generated, as well as photosensitiser bearing two reactive esters (Vrouenraets et al, 1999).
While conventional cloning methods using restriction enzymes and polynucleotide ligase are adequate for most DNAs, fragments made by the polymerase chain reaction are difficult to clone because the amplifying DNA polymerase tends to add untemplated nucleotides to the 3'-termini of the amplified strands.
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Precisely what level of heat causes the reaction is difficult to pinpoint.
At the cathode, the electrons and the hydrogen ions combine with oxygen molecules to form water (and also release some heat in the process).The principle, therefore, is quite simple, but the chemical reaction is difficult to produce.
In the condition of neutral pH, metasilicate formation was mainly from the hydrolysis of SiO2, but the reaction was difficult.
In these processes, α-Fe2O3 NPs could be used in the form of a fine powder or crystals dispersed in water, but it is vital to know that filtering these NPs following reaction is difficult and costly.
Addition of CO2 was beneficial to the formation of metasilicate, and the release process of metasilciate accorded with Boltzmann equation in the condition of neutral and alkaline pH. (4) In the condition of neutral pH, metasilicate formation was mainly from the hydrolysis of SiO2, but the reaction was difficult.
"The worst fears of Putin are becoming a reality and his reaction is difficult to predict and could be one of desperation," said Igor Sutyagin, Russian studies research fellow at Britain's Royal United Services Institute.
However, understanding whether the antibodies are associated with specific neurological symptoms or are only marker of anticancer immune reaction is difficult [16].
Distinguishing inflammation due to a bacterial biofilm from a low-grade hypersensitivity reaction is difficult.
19, 20 However, the acylation of the Hmb derivatives can be problematic, and the reaction is difficult to follow and often requires nonstandard acylation conditions.
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