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The U.S. and global stock markets nosedived following Bernanke's words, a fearful reaction amplified by worrisome signs that China's economy is slowing.
The 5-HTTLPR region was polymerase chain reaction amplified using the oligonucleotide primers SLC6A4_SE (5′- CTCCTAGGATCGCTCCTGCATC-3′) and SLC6A4_AS (5′-GGACCGCAAGGTGG GCGGGAGGCTTGGAG-3′), resulting in amplicons of 294 base pairs for the S variant and 337 base pairs for the L variant.
Each reaction amplified only messages produced from the given promoter analyzed.
As shown in Fig. 1D, this reaction amplified two bands of 633 and 847 base pairs, respectively.
The primers used in the PCR reaction amplified sense or antisense RNA corresponding to the sense open reading frame.
Following hybridization, the probes were ligated with ligase 65 and polymerase chain reaction amplified utilizing FAM-labeled M13 primers.
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In patient 1, the IgA reactions resulted in 1 amplified product (IGHV 1) and the IgG reactions amplified two products (IGHV 1 and 7; Supplementary Figure 3).
After completion of the PCR reactions, amplified products were separated by electrophoresis in agarose gels containing ethidium bromide (0.5 μg ml−1).
The peptide sequence data are consistent with the DNA sequence of the polymerase chain reaction-amplified fragment of the variant globin gene, which shows the insertion of the triplet codon--GAA--into the mutant alpha-globin gene.
MC1R was sequenced using a 1125-bp polymerase chain reaction-amplified fragment to cover the coding region.
The two forward primers were not multiplexed in a single reaction, but amplified separately for each of the 16 samples, necessitating 32 total reaction cocktails, each with a unique barcoded forward primer.
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