Sentence examples for reaction absorbance from inspiring English sources

For the LiP activity, 400 μL of the supernatant was used for the reaction; absorbance measurements were performed at a wavelength of 310 nm.

where C0 represents the initial concentration, ΔC represents the changed concentration, C represents the reaction concentration, A0 represents the initial absorbance, ΔA represents the changed absorbance, and A represents the reaction absorbance of the MO at the characteristic absorption wavelength of 464 nm.

After a 30-min reaction, absorbance was determined.

After adding the stop solution to halt the enzymatic reaction, absorbance at 450 nm was measured using a spectrophotometer.

The absorbance of the reference was subtracted from the reaction absorbance, prior to determining the enzyme concentration.

Cells were co-incubated for 4 h at 37°C, the supernatants were collected and the LDH release was evaluated by a colorimetric reaction (absorbance at 490 nm).

After 10 min reaction, the absorbance was measured at 700 nm against a blank in a spectrophotometer.

NaOH was used to stop the reaction, and absorbance at 405 nm was measured (PowerWave x340 Microplate Reader; BioTek Instruments).

After incubation at 25 °C for 40 min, the reaction mixture absorbance was measured at λ = 420 nm against distilled water as a white, using a spectrophotometer.

Enzyme activity was evaluated as the amount of nitrophenol released through the enzymatic reaction, and absorbance was recorded using a microplate reader (Bio-RAD 680, Hercules, CA, USA) at 405 nm.

The kinetics of formation of AuNPs was followed by UV-vis spectroscopy, and the spectra obtained are shown in Figure 2. It is observed that with the progress of the reaction, the absorbance intensity at 546 nm increases constantly with time, while the absorption peak of AuCl4- (initial) at about 290 nm disappeared suddenly after about 1 h of reaction time.