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After incubation, the reacted solution was heated at 85 °C for 15 min, then rapidly cooled.
The alkyl dihydrocaffeate esters were obtained by extracting each reacted solution in diethyl ether.
The reacted solution is allowed to dry in the hot air oven to yield pale white precipitates.
The effect of O2 in the reacted solution was most pronounced with NO3- in the presence of Ni0 (run 70).
The absorbance of reacted solution was measured at 540 nm on spectrophotometric plate reader Multyscan (Labsystem, Finland).
For each given irradiation time, about 3 mL of the reacted solution was withdrawn and centrifuged at 12,000 rpm for 30 min to remove the photocatalyst.
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The reacting solution is chilled to precipitate the PETN, which is filtered out, washed, dried, and recrystallized to produce a colourless crystalline material that is generally stored and shipped as a mixture with water and alcohol.
To deduce kinetic parameters for the reactions of carbon dioxide (CO2) in carbonate solutions the physical solubility of CO2 into the reacting solution is needed.
After degradation, reacting solution was centrifuged for 10 min at 13,000×g.
In the initial stage, the reacting solution consists of Cl- ions and Ti precursors.
The temperature of the reacting solution was controlled, using an automatic circulation thermostat.
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