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The reacted products were purified using Ethanol Precipitation and run using Automatic Sequencer 3730 × l (Macrogen, DNA sequencing, USA).
To test for whether direct interaction between NAC and BiQs occurs, we performed mass spectrometry on BiQ7 (MW 398 Da) and NAC (MW 163 Da) both separately and after co-incubation (predicted reacted products at 526 Da or 561 Da).
Radioactivity of reacted products was measured with a liquid scintillation counter as described in the Materials and methods.
In contrast, the same reactions with the more congested homologue 7b yielded a mixture of partially reacted products and none of the desired tetra-acylation product.
The toluene phase (500 μL) was mixed with 3 mL cocktail and the radioactivity of reacted products was measured with a Tri-Carb 2100 liquid scintillation counter (Packard Instrument, Connecticut, USA).
Cycling conditions were 94°C for 3 min, and 35 cycles of 94°C for 30 s, 50°C for 40 s and 72°C for 60 s and a final extension step of 72°C for 7 min. Sequencing was conducted under BigDye™ terminator cycling conditions, the reacted products purified using ethanol precipitation and run using an Automatic Sequencer ABI 3730xl.
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The reacted product was identified as Ni2Si.
Thus, diffraction signal mainly demonstrate an amorphous phase in the longer time reacted product.
We are also able to correlate the reactivity and functionality of the plasticizer to the crosslink density in the reacted product.
B4C could consume the oxygen diffused into the coating and the reacted product B2O3 with a better fluidity at 800 °C could effectively heal cracks and pores in the coating to improve the oxidation resistance property.
The model is based on the principles of the "unreacted-core" systems, typical of chemical engineering processes, in which the reacted product remains in the solid as a layer of inert ash, adapted for the specific case of carbonation.
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