Sentence examples for reaching passage from inspiring English sources

Cells were used for experiments upon reaching passage 6 (P6).

Extended cultures were also protracted until reaching passage (P) 4. At each passage, cells were counted using 0.4%% trypan blue (Biochrom AG) exclusion, to evaluate viable cell expansion.

The cells were maintained at sub-confluent densities and used for experiments when reaching passages 3 or 4.

Cells were cultured at a density of 10,000 cells/cm2 with Ham/F12 media, supplemented with 10% fetal bovine serum, 50 µg/ml gentamicin, and 2.5 µg/ml amphotericin B. When cells reached passage two, they were shipped for further culturing and experiments.

Human dermal microvascular endothelial cells were used in the experiments until they reached passage 6.

For each experiment, cells were thawed and used before they reached passage ten.

Thus, the s.c. xenografts have presently reached passage 5, whereas both i.p. models are in passage 14.

When cells reach passage 4 we calculate doubling cells (DC) follow the formula: DC = Log10(Pi+1/Pi)/(log210) where Pi means number of cells in each P in vitro [ 18].

Cells were cultured until homogeneous morphology of cells was reached (passage 3 4) because LASP-1 belongs to a group of several differential expressed proteins that are upregulated after later passages (Sun et al, 2006).

To further investigate whether the altered methylated TRF2 staining might be a general feature of replicative senescence, we passaged young IMR90 (p22) cells continuously every four days for 72 days until they reached passage 40 (Fig. 4A).

The β-galactosidase staining, performed to evaluate senescent cells, showed that cells cultured in Quantum 333 underwent senescence at passage 17, while those cultured in αMEM supplemented with 10% FBS reached passage 26 before growth arrest– 4(d)).