Caco-2 cells undergo enterocyte-like differentiation upon reaching confluence.
RA-FLS grew out of the tissue and colonized the plastic dishes until reaching confluence.
Differentiated cells were transferred to DMEM containing 2% horse serum upon reaching confluence.
However, upon reaching confluence, we frequently observed clusters of cells resembling classical neural rosettes (Supplementary Fig. 5c, circles).
After reaching confluence, these cells were trypsinized, centrifuged, suspended in media, and counted.
The uptake experiments were conducted after cell reaching confluence in a chamber slide.
Proliferating cells were passaged before reaching confluence.
Adherent cells were harvested by trypsinization before reaching confluence.
Cells were maintained and passaged upon reaching confluence by standard cell culture techniques.
3T3-L1 preadipocytes were seeded in 6-well plates and transfected two days after reaching confluence.
After reaching confluence, cells from a single well were expanded for maintenance under neomycin selection.
