The samples reached the selected temperature with a thermal gradient of 5°/min and were kept at the annealing temperature for 60 min, then gradually cooled down to room temperature.
Animals were maintained on this 1∶1 schedule until they reached the selected criterion — namely, until they were able to obtain ≥20 pellets for 2 successive sessions.
By the 5th session, all PHY and SOPHY animals had already reached the selected criterion, whilst 42±12.5% of AL and 54±16.4% of SO mice reached criterion only during this session.
Trained community health workers (CHW) reached the selected household and approached the head of the family.
Because normal distribution of biological values was not reached, the selected parameters were categorized: cutoffs values were determined by optimal likelihood ratios of individual receiver operating curve (ROC) analysis, or according to the manufacturer's recommendation for PCT.
Similarly, five additional genes were significantly altered across both males and females in the dose response study; however the magnitude of these changes reached the selected threshold in only male (Hunk, P2ry4, Sun3 and Ugt1a9) or female liver (Rcan3).
