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The mice with cell line N tumor fragments reached humane endpoints faster than those with line B tumor fragments, and all the mice were euthanized at 9 weeks after implantation (Figure 4).
These mice eventually succumbed from infection or had to be taken out of the experiment because they reached humane endpoints.
Two non-vaccinated controls reached humane endpoint at week 12 post-infection due to sustained weight loss, and one BCG-vaccinee was withdrawn at week 14 post-infection after showing signs of distress.
Mice that received a single shot of PBS, wtMVA or the lowest dose of MVA-HA-VN/04 (103 pfu) did not survive past day 7 p.i. with influenza virus A/VN/1194/04 and A/IND/5/05, and most of them reached humane endpoints six days p.i. (Table 2).
By 260 days, 58% of the B2; Ptk6 −/− animals remained tumor free, whereas all B2; Ptk6+/+ mice had reached humane end points and mice were killed.
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And even if the return to growth this year convinces investors to put their money in Greece, it will take years for unemployment to reach humane levels.
Animals reaching humane endpoint before day 23 (n=7) (predefined as limb use score 0) were euthanized by decapitation following brief exposure to isoflurane, and the behavioral scores from the last day carried forward for data analysis.
Mice treated with 8-MeXAA or 7,8-MeXAA at doses above 50 mg kg−1 begin losing weight 14 days after treatment and reach humane ethical endpoint shortly thereafter, and 50 mg kg−1 was used as the MTD for long-term survival experiments.
The remaining three negative control mice reached a humane endpoint and were thus euthanized before SPECT/CT imaging.
SPECT/CT imaging was initiated 8 weeks after orthotopic implantation since some mice appeared to have reached the humane endpoints around this time point.
Compared to immunocompetent B6 animals, B6.RAG-2γc−/− mice are highly susceptible to infection with a low dose of HKx31 (102 PFU) and all mice reached the humane end-point for culling by day 10 post-infection (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com