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This objective is reached by replacing conventional urban drainage pipes with swales, filter strips, wetlands and ponds as collection, storage and treatment systems designed for natural processes.
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Adipogenic differentiation was induced 2 days after cells reached confluency by replacing the medium with differentiation medium (DMEM containing 1 μM dexamethasone, 0.5 mM methylisobuthylxanthine, 10 μg/ml insulin, 10 μM troglitazone and 10% FCS).
Upon reaching ∼85% confluence, cells were differentiated by replacing their GM with differentiation medium (DM), which is equivalent to the GM formulation but containing only 2% FBS.
Verdicts are reached by majority.
He was reached by cellphone.
Once the cells reached the required confluency, two sets of triplicate wells were induced to differentiate by replacing the culture medium with the specific differentiation medium.
By replacing 30% of the recycled CBM-lacking enzymes the hydrolysis yield reached 45.9% of total carbohydrates, 88% of the reference.
Transfected myoblasts were stimulated to differentiate by replacing the growth medium with DMEM supplemented with 2% HS, when the cells reached confluence.
Hemodilution was performed by replacing blood with HES solution (6 % Hydroxyethyl Starch 130/0.4, Voluven, Fresenius Kabi) till hematocrit levels reached 20%% (T1), 15%% (T2) and 10%% (T3).
This effect could be incorporated in our model by replacing the constant thermogenesis parameter K with a decreasing function of temperature until thermo neutrality is reached.
Finally, by replacing the values from Table 2, as in the example above, and rearranging terms, expression (36) is reached.
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