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The rDEN2Δ30 virus reached a virus titer of 6.6 log10PFU/ml in Vero cells.
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Site directed mutagenesis was used to introduce a Ser→Leu mutation at amino acid 158 of NS3 in rDEN2Δ30-7169, and the rDEN2Δ30-4995 virus was recovered in C6/36 cells and propagated in Vero cells reaching a virus titer of 6.2 log10PFU/ml (Table 1).
While the latter two viruses had detectable viremia in only 50% of monkeys, a mean number of viremic days of less than one day, and a mean peak viremia of less than 1.0 log10PFU/ml [ 13, 14], the rDEN2Δ30 virus infected 100% of the rhesus monkeys and reached a peak virus titer of 1.7 log1.7FU/ml.
The stock reached an infectious virus titer of 106.9 median tissue culture infectious dose (TCID50) per mL.
When the tumors reached a certain size, the virus was injected into the tumor.
First, both viruses reached a titer over 6.0 log10PFU/ml in Vero cells that would permit economical manufacture.
Swans were particularly susceptible to avian flu, so acted as sentinels that the virus had reached a new area, he added.
Professor Heymann, who worked as an epidemics expert in sub-Saharan Africa during the first ever Ebola outbreaks in the 1970s and '80s, said this would not be the first time the virus had reached a major European aviation hub.
Virus production reached a plateau at day 2 pi for the two MOIs used (Figure 2A).
Putting in the final piece of the puzzle, Sharp said the virus must have reached a major city to start the AIDS epidemic.
The seroprevalence of these viruses had not reached a plateau by the end of study; thus, some infections are expected to occur later than 13 years of age.
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