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Initial spreading was defined as the first time point where contact was initiated and (i) contact was maintained for at least 45 s, and (ii) contact area reached a minimum level of 7.8 μm (corresponding to 500 pixels).
The column was washed with lysis buffer until the A280 reached a minimum level where no further was observed (approximately 200 mL) and the PFTase enzyme was eluted using the above lysis buffer supplemented with 250 mM imidazole.
The serotypes 3a, 3b, 3c, 4c, 4e, and 7 and nontypable samples, with rough R-forms, reached a minimum level (119 2.06%), and the isolation of the serotype 4e in milk samples from São Paulo and unprocessed meats (beef and chicken) from Rio Grande do Sul is also noteworthy.
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It would also raise enrollee premiums for Medicare Part B — which now cover 25 percent of the cost of coverage for physician services — by 2 percentage points every year for five years until they reach a minimum level of 35 percent of those costs in 2019.
The transmitted data sequence consists of 16 symbols, which are repeated until the capacitor reaches a minimum level of charge.
The requirement that a just society ensure that every citizen reaches a minimum level of each capability may be far less demanding than the requirement of equal capabilities.
However, in the only available longitudinal study, a decrease in BFMI has been observed during adolescence in American boys, reaching a minimum level at the age of 15 years old [26].
The consequent stochasticity of the output CEA value, quantified by CV, monotonically decreases with increasing amount of input and reaches a minimum level as the number of CC molecules is above 60 for all the eight conditions.
We discovered seven flanking region pattern associations in the Broad dataset which reached a minimum significance level of p ≤ 0.05.
These CSF lipid levels reached a minimum at 7 months, a return to baseline at 15 months, an overshoot that peaked at 24 months and a drop towards baseline at 36 months.
Transfecting siBnc1-s into HaCaT cells decreased basonuclin mRNA level, which reached a minimum at 12 h post transfection but recovered somewhat at 24 h (Fig. 2A, RT-PCR) and maintained at a low level during the rest of the experiment.
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