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In this case, the extracted soft clip contains untemplated sequence and when re-aligned to the reference, the re-aligned soft clip sequences will themselves be soft-clipped (by Θ nt).
Sequences were re-aligned [ 77], and alignment gaps were removed [ 78].
The new sequences (31) were re-aligned to the original multiple alignment using T-coffee.
The final 'no-gap' set was re-aligned producing the final multiple alignment.
To improve the quality of local alignments, each upstream region was re-aligned using ClustalW [ 30].
These files were then re-aligned using MUSCLE to improve subfamily alignments.
In 1931, Route 6 was re-aligned south of Canton, and the former alignment was designated as Route 96.
In this case, the re-aligned soft clips will be placed on locus A and C omitting B as soft clips of the re-aligned locus.
Each group was re-aligned with Probcons, and each of these sub-alignments was manually adjusted.
Split read clustering: This works on the re-aligned soft clip file and produces a data structure of split-read clusters that share the same anchor and re-aligned regions.
The reduced alignments were then treated as separate profiles and re-aligned using MUSCLE [ 40].
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