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After a 7 day fast, one additional unfed group was re-fed and sampled at 3 h post-re-feeding.
We also measured insulin response to re-feeding.
Gonads were dissected immediately before re-feeding and at 1-hr intervals 2 6 hr after re-feeding.
abu-14 mutant animals were assessed at 72 hours after re-feeding.
On the other hand, autophagic AF cells could be rescued by re-feeding with FBS.
We therefore tested whether longer periods of starvation would delay mitotic re-entry upon re-feeding.
We next challenged wild-type FVB mice with an overnight fast followed by re-feeding.
For example, muscle loss upon fasting is rapidly and completely reversed upon re-feeding.
The level of both proteins increased in parallel, shortly after re-feeding.
To compare meiotic entry of transient progenitors under fed versus starved conditions, we re-fed starved animals and restricted cell movement during re-feeding; we then compared meiotic entry after re-feeding to meiotic entry after continued starvation.
Although long term data are not available for our cohort, only seven (10.3%) were readmitted for re-feeding.
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