At a dilution of 1 200, 50 μl of urine sample was assayed directly; where necessary, samples were re-assayed at dilution of 1 400.
Of the total number of serum samples, 135 (5%) required re-assay for sFlt1, 29 (0.9%) for sEng, and 33 (1.2%) for PlGF.
One approach to addressing this conflict is to employ a third and independent methodology, such as SSCP (single-stranded conformation polymorphism), to re-assay these regions.
To confirm that the growth on selective plates was due to this interaction, yeast was cured of one or both plasmids and re-assayed.
On the basis of these initial assays, samples containing a level of FMDV RNA which were expected to yield, in this standard diagnostic assay, Ct values of 15, 20, 25 and 30 respectively were prepared and re-assayed.
For a comparison of population serological profiles over time, a cross-sectional survey was performed in Brefet village in the Western Region in December 2009 and samples assayed alongside those sampled in December 2006 [25] that were re-assayed here.
Samples which tested negative with the commercial kit and positive by L-asparaginase ELISA were re-assayed with a second commercial kit for Helicobacter pylori IgG (commercial kit 2).
For reconfirmation, a set of 96 cDNAs chosen from among the top afa scores was re-assayed in quadruplicate, and raw luciferase values were normalized to empty vector controls.
To validate these potential INK4a regulators, a subset of the top activators was re-assayed in quadruplicate, and screened against the promoterless luciferase reporter backbone (pGL3) to eliminate nonspecific activators.
In fact, the re-assay of baseline insulin levels with the same method used in later examinations may reduce the measurement error and strengthen our study.
