Figure 5(A) shows white-light images of cells attached to coverslips, whether 16 h post-transfection (adherent) or non-adherent cells collected at 16 h and re-adhered to coverslips overnight (re-adhered).
And that's because if they just loosen it up, the bone regrows and re-adheres the stapes from vibration.
Next, the warm adhesive, a 30% hide glue in distilled water, was applied to the surfaces with a brush, and the pieces were re-adhered to the statuette.
The cells were then incubated overnight to allow the cells to re-adhere to the plate.
I was able to repair scarf tears, rejoin the separated parts, and/or re-adhere the page to the mount with wheat starch paste.
When you examine every square inch of a painting, re-adhere loose paint, fill in losses, and retouch missing sections, you get to really know a painting and appreciate good craftsmanship and see the effects of bad craftsmanship.
After two weeks, EBs were collected, transferred to tissue culture plates where they re-adhered, and ultimately passaged in fibroblast medium.
Figure 1D shows that the cells from the spheres re-adhere and morphologically resemble the parental USSCs in the presence of fetal calf serum, or when the surface is coated with extracellular matrix proteins.
Both spheroid and differentiated cells were harvested, disaggregated, plated at 10 000 cells per well in 96-well plates, and cultured in either CSC or serum-containing media, respectively, for 24 h to allow the differentiated population to re-adhere, whereas ⩾90% of the spheroid-derived cell populations remained as single cells in suspension with no evidence of differentiation detected.
However, we also observed expression of T/Bra and Eomes, which are genes associated with Primitive Streak and endodermal fates (the sharp decline in T/Bra levels on day 1 most likely represents the failure of cells expressing this gene in BMP4+LIF conditions to re-adhere when reseeded).
We demonstrate that fragments of tissue generated by mesodissection can be re-adhered to slides and used for FISH analysis.
