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where RCL 1 = S 1 ⋅ ACL 1 − A 0 − CL 1 max Open image in new window N 1 = 1 + C L 1 max RCL 1 Open image in new window.
The lift coefficient in the pre-stall regime is given by: if α ≥ A C L 1 = S 1 ⋅ α − A 0 − RCL 1 α − A 0 AC L 1 − A 0 N 1 Open image in new window if α < A 0 C L 1 = S 1 ⋅ α − A 0 + RCL 1 − α + A 0 AC L 1 − A 0 N 1 Open image in new window.
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where RCL 2 = 1.632 − C L 2 max Open image in new window N 2 = 1 + C L 2 max RCL 2 Open image in new window.
Removal of RCL (∆373 380) also results in insufficient secretion due to impaired PEDF protein transport from the endoplasmic reticulum to the Golgi [ 238].
The C-terminus (residues 415 418), RCL (residues 373 380), and hydrophobic core (Asn Thr) corresponding to β-sheet B were found to be essential for human PEDF secretion [ 238].
For example, the mammalian non-inhibitory serpins corticosteroid binding globulin (CBG; SERPINA6) and thyroxin-binding globulin (TBG; SERPINA7) bind steroid hormones in the blood and release these compounds at specific sites via a mechanism involving cleavage of the RCL [ 19, 20].
Simvastatin was purchased from Pfizer (New York, NY, USA), and RCL-2 solution was purchased from Alphelys (Toulouse, France).
Tissue samples were immediately washed with normal saline and preserved in chilled RCL-2 solution, which was then transferred to −80°C until RNA was extracted.
Laser powers of 3-12 mW at 532 nm (CrystaLaser GCL-025-L-0.5%) for SCION(Alexa555) and 635 nm (CrystaLaser RCL-025-640-S) for SCION Cy5.5) were used to excite the particles.
405-nm (CUBE 405-50C; Coherent, Santa Clara, CA), 460-nm (Sapphire 460-10; Coherent), 532-nm (GCL-200-I; CrystaLaser, Reno, NV), and 657-nm (RCL-300-656; Crystalasers lasers were introduced into the sample through the back focal plane of the microscope.
A solid-state long coherence length laser at 785 nm (CrystaLaser RCL-785-080-S) was coupled to a 62.5 μm multi-mode gradient index fiber and delivered to the tissue.
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