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Samples were applied to ultracentrifugation (48,900 rcf, 1 hr, 4°C).
45 ml of supernatant was applied to ultracentrifugation (16,500 rcf, 1 hr, 4°C).
A small pellet was observed and the ultracentrifugation step was repeated (46,000 rcf, 1 hr, 4°C).
After stopping digestion with a serum- and calcium-containing buffer, cells were washed with PBS and collected by centrifugation (180 rcf, 1 min).
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A pellet was obtained by centrifugation (rcf 11,000 g) and then washed twice with 75% ethanol.
A size separation was performed by centrifugation (1,500 RCF, 5 min) in order to achieve a narrow particle size distribution.
The micro-tube was centrifuged (800 rcf, 5 min) and the supernatant was removed; then, the pellet was resuspended in serum-PBS (500 µL).
RCF, NIH3T3 fibroblasts, A431 keratinocytes, L6 myocytes, and HepG2 hepatocytes were seeded and allowed to grow for different time period as shown in respective figures.
Tubes were centrifuged at 41,500 rcf (20,000 rpm) for 10 min at 4°C to pellet membranes.
Overnight YPD-grown cultures were centrifuged (3,220 rcf; 5 min; 4°C) and washed with sterile water.
There was a significant negative association between preintervention uracil misincorporation and RCF (P<0.01 r=−0.5).
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