This novel CL approach utilized rolling circle amplification (RCA) as a signal enhancement technique and the 96-well plate as the immobilization and separation carrier.
After a third round, the signal on RCA-A further increased as expected and it was 15-fold higher than the background, and 25-fold higher on ricin, which would be in agreement with the fact that whole ricin had been utilized for panning.
RCA-MO and RCA-VA mean the RCA from Montreal and Vancouver, respectively.
Moreover, by making use of carboxyl-coated polystyrene microspheres, 3′ NH2 immobilized aptamer and 3′ NH2 modified primer DNA are loaded through amidation onto the sensing layer for further rolling circle amplification (RCA) process to amplify the SPR signal.
Recently a new method for SNP detection from genomic DNA based on DNA ligase-mediated single nucleotide discrimination and signal amplification by Rolling Circle Amplification (RCA) has been described [ 6- 10].
To further improve detection performance, rolling circle amplification (RCA) was employed to enhance the fluorescence signals.
Finally, someone from RCA just wanted to apologize to me on behalf of RCA.
This sensing method demonstrates a new concept of IB-RCA amplification even in a simple way to efficiently transduce the fluorescence signal, accomplishing the highly sensitive and selective detection of cancer gene.
A sensitive protocol for surface enhanced Raman spectroscopy (SERS) detection of thrombin is designed with R6G-Ag NPs as a signal tag by combining DNAzyme assistant DNA recycling and rolling circle amplification (RCA).
In this work, we developed a cascade signal amplification strategy for detection of IgG antigen by combining the rolling circle amplification (RCA) technique with oligonucleotide functionalized carbon dots (CDs), based on a paper-based electrochemiluminescence (ECL) origami device (PECLOD) for the first time.
