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The raw ratio of bachelors to bachelorettes varies with age.
First, we used defined Minor Allele Frequency (MAF) to determine the raw ratio of three genotypes as Major Allele Homozygous (0), Heterozygous (1), and Minor Allele Homozygous (2).
The background subtracted values were then used to calculate the ratio of medians (Cy5/Cy3) which corresponds to the raw ratio value for each gene.
This has theoretical advantages over the original raw ratio, because it incorporates an estimate of possible statistical errors in the analysis by giving a p-value attached to each genomic change within a SW-locus.
Known clade II copy number controls were included in every experiment, and used to calibrate the raw ratio results, to minimize the effect of heteroduplex formation, which consistently underestimates the amount of cut fragment by being refractory to restriction enzyme digestion.
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All data files for raw ratios of ssDNA or breakage are available in File S1.
A fractionation-corrected 98/95Mo ratio for the standard was determined by measuring double-spiked aliquots and reducing the raw ratios.
This plot shows raw ratios, but ratios normalized by intensity, a common normalization method, show a similar relationship (not shown).
Between the dietary groups, the hard food eaters have significantly higher mechanical advantage (MA) values for all four MA measures (based on both the raw ratios and residuals from the regression of numerators on the denominators of these ratios, using both the Student's t-test and Kruskal-Wallis tests: p < 0.01).
A duplication is more likely than a triplication given the change in raw ratios from the two PRT assays used (approximately 1 1.5 2 for homozygote, heterozygote and homozygote duplication respectively) and this is consistently seen when the HPR duplication is inherited in trios.
For the ionic liquid based ultrasonic assisted extraction, optimum process was set as below: [Omim]Br concentration, 4.2 mol/L; liquid-raw ratio, 30 mL/g; extraction time, 90 min; ultrasonic power, 250 W.
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