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An additional table shows the raw quantification results (see Additional file 7 ).
Such raw quantification is usually followed by normalization for reference coding sequence length [ 45].
Raw quantification and background noise values were represented for each chip (data not shown).
Raw quantification data and their processing to NAIs are summarized Excel files.
In addition, the analysis of raw quantification cycle (Cq) values of each gene was used to evaluate their stability.
Raw quantification cycle (Cq) values were transformed to relative quantities using the software program geNorm, version 3.4 [ 31] http://medgen.ugent.be/~jvdesomp/genorm/.ugent.be/~jvdesomp/genorm/
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See Additional file 7 for raw quantifications.
The raw quantifications were calculated using the iQ5 Optical Detection System software and normalized to the 18S rRNA values for each sample.
Image acquisition, raw signal quantification, data preprocessing, and quantile normalization were performed with Illumina and BioArray Software Environment BASEE) (SCIBLU) software as described previously [20].
The Illumina and BioArray Software Environment BASEE; SCIBLU) was used for image acquisition, raw signal quantification, data preprocessing, and quantile normalization as described previously [11].
Results are expressed in raw relative quantification (RQ) ± standard errorrs.
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