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Raw nucleotide sequences were filtered, aligned, trimmed and translated using pre-specified criteria applied uniformly so that all V3 sequence forms included in subsequent analyses spanned the complete V3 loop and the two proximal N-linked glycosylation sites.
All raw nucleotide sequence reads are deposited at the NCBI Sequence Read Archive SRAA).
Raw nucleotide bases were called with RTA 1.17.21.3 and converted to the Fastq-format files with bcl2fastq 1.8.3 (Illumina Inc).
Raw data generated by the sequencer were converted to raw nucleotide reads with Illumina GA Pipeline 1.6.
To estimate the genome coverage, raw nucleotide reads from both Illumina and 454 sequencing platforms were trimmed and filtered.
This framework ('MLTreeMap'; http://mltreemap.org/) uses raw nucleotide sequences as input, and includes hand-curated, extensible reference information.
Similar(42)
But what is, perhaps, most noteworthy is that the starting point for the project was not the raw nucleotides (the chemical letters of which DNA is composed), but a set of pre-assembled "cassettes" of DNA that the team had ordered from commercial suppliers.
At this point, the unigenes (contigs plus singletons) were available in three forms: raw nucleotides, predicted amino acids and nucleotide coding sequences (cds).
As a way of summarizing differences between sequence clusters, raw pairwise nucleotide and amino acid differences and distances were calculated based on the coding region for each category of amplicon (Figure 6).
The increase and decrease in the number of chromosome molecules at the equilibrium is generally opposite to that of the raw materials (nucleotide precursors' precursors, cyan dots).
The raw single nucleotide polymorphism (SNP) data for human and mouse come from dbSNP (Sherry et al. 2001) release 130 and 128, respectively.
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