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This represents trogocytic intermediates in which transfer but not internalization of RTX has occurred; these may either be free RAW cells that have detached a piece(s) of Ramos cells, or the RAW cell halves of RAW-Ramos conjugates that are dissociated in the process of detachment and resuspension of the RAW cells (few if any conjugates remained intact after RAW detachment (Figure S1A)).
RAW cell adherence assays were based on the RAW cell phagocytosis procedure with the following modifications.
Nanoparticles uptake by RAW cell over 4 h time period was monitored by light microscopy.
In addition, extensive uptake was observed in mouse macrophage-like RAW cell, suggesting that nanoparticle uptake is highly dependent on the cell type.
Aim of this work was to establish the optimum operating conditions for the extraction and recovery by cationic reversed micelles of commercial glucose oxidase (GOX) from Aspergillus niger, in view of possible application to raw cell homogenates.
RAW cell media was replaced with phenol red-free HEPES-buffered RPMI (HPMI) with serum (Wisent).
RAW cell events were analyzed by an external RTX (anti-human) versus total RTX plot.
RAW cell phagocytois assays were based on the S2 cell procedure with the following modifications.
In either case, appearance of this population represents a first step in which RTX becomes associated with the RAW cell prior to its internalization.
(See Dataset S1 for an example of the custom Excel macro used to generate DNA distribution plots from raw cell cycle data output).
We also monitored the total number of C. albicans phagocytosed and used this to calculate the number of C. albicans cells phagocytosed per RAW cell scored.
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