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The rats were then decapitated.
Group 1 rats were then subjected to sham operation.
The rats were then killed at different times.
Rats were then killed and organs collected for 109Cd assays.
The rats were then randomly divided into two groups.
The rats were then sacrificed at different time points.
Some rats were then fed 2-ACB's, while others were not.
The rats were then removed and put back in the housing cage.
Rats were then assigned a chamber to be paired with morphine in an unbiased counterbalanced design.
Rats were then perfused with 4% paraformaldehyde and the spleens and brains were removed and cryoprotected in 30% sucrose.
The rats were then subjected to a 20-day extinction phase where lever presses had no programmed consequences.
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