On day 14, first rats were tested on the ICSS task.
Thereafter, all rats were tested under each noise condition.
Middle panel: Rats were tested for morphine preference (CPP tests) every 1 2 weeks throughout dependence and abstinence.
All rats were tested for total 10 min, with the location of the bottles swapped every 30 s.
Here, rats were tested after 1 week of dependence, 1 week of abstinence, and 3 weeks of abstinence.
The nondeprived rats were tested under the progressive ratio procedure after 23 weeks of continual ETOH operant exposure.
Rats were tested at the University of Maryland School of Medicine in accordance with School of Medicine and NIH guidelines.
After 10 weeks of daily operant self-administration, rats were tested in a progressive ratio paradigm.
The newborn rats were tested following similar exposure beginning on the 2nd day after birth.
Rats were tested in two set-shifting tasks following quinolinic acid injections bilaterally into the DMS.
10 days after surgery rats were tested for memory of the shock context.
