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The eyes of E18 and P1 rats were fixed in 4% paraformaldehyde for 24 h.
Livers from 5 day-old rats were fixed in 0.2% glutaraldehyde −2% paraformaldehyde in PBS 0.1 M, dehydrated and embedded in paraffin.
Following anesthesia rats were fixed by transcardial perfusion with 2.5% glutaraldehyde in 0.1 M Na-cacodylate buffer, pH 7.6 containing 2% polyvinylpyrrolidone and 0.05% CaCl2.
Rats were fixed in Bouin solution (750 mL of saturated picric acid, 250 mL of formaldehyde 37%, and 50 mL of glacial acetic acid) or 4% paraformaldehyde (PFA) using vascular perfusion, and the samples were dissected and post-fixed by immersion (12 h).
Rats were fixed by perfusion with saline followed by 4% paraformaldehyde.
Rats were fixed in vivo by retrograde perfusion, as described in [ 7, 13].
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The 6 conic restrainers (Fig. 1B top) for the immobilisation of the rats are fixed in such a way that the rats' brains are always positioned directly below the lower end of the gap between the ribs (Fig. 1B bottom).
Rats are fixed in a prone position during scanning.
pWAT of 21-day-old BPA-exposed and control rats was fixed in a paraformaldehyde solution for 24 hr and embedded in paraffin.
Brains for E16 or P6 Sprague Dawley (SD) rat were fixed with 4% paraformaldehyde, followed by addition with 10% sucrose.
The four limbs of the rat were fixed supinely after it was anesthetized and the two-dimensional ultrasonography was performed after penis was fully exposed.
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