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To identify the neural site for kisspeptin-GPR54 signalling to control pulsatile LH secretion, a separate group of rats was implanted with bilateral guide cannulae (22-gauge; Plastics One) directed at the GnRH-rich areas of the mPOA or the ARC for microinfusion of kisspeptin or its antagonist.
To evaluate the inhibitory effect of the selective kisspeptin antagonist on pulsatile LH secretion, a group of rats was implanted with an icv guide cannula (22 gauge; Plastics One, Roanoke, VA, USA) directed towards the left lateral ventricle, the co-ordinates for implantation being 0.6 mm lateral and 1.5 mm posterior to bregma, and 4.5 mm below the surface of the dura.
A group of naive rats was implanted with guide cannulae and received an intracerebral microinjection of 2 microliters of ACSF and served as a control of the intracerebral drug microinjection.
Similar(57)
To establish ANG II-induced hypertension, 7 days after the implantation of a radio-transmitter into the abdominal aorta, male Wistar rats were implanted with an ANG II-containing Alzet osmotic pumps subcutaneously, which delivered at a rate of 200 or 50 ng/kg/min.
Rats were implanted with microdialysis guide cannulae as described above.
Non-dependent rats were implanted at the same time with placebo pellets.
Rats were implanted with a pair of silicon pocket electrodes as described above.
Male Wistar rats were implanted with an i.t.
Rats were implanted with electrodes for electrocorticography and electromyography.
Rats were implanted with telemetry transmitters with electroencephalogram/electromyogram electrodes.
Male Wistar rats were implanted with either one or two i.t.
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