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pWAT of 21-day-old BPA-exposed and control rats was fixed in a paraformaldehyde solution for 24 hr and embedded in paraffin.
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The eyes of E18 and P1 rats were fixed in 4% paraformaldehyde for 24 h.
Livers from 5 day-old rats were fixed in 0.2% glutaraldehyde −2% paraformaldehyde in PBS 0.1 M, dehydrated and embedded in paraffin.
Following anesthesia rats were fixed by transcardial perfusion with 2.5% glutaraldehyde in 0.1 M Na-cacodylate buffer, pH 7.6 containing 2% polyvinylpyrrolidone and 0.05% CaCl2.
The 6 conic restrainers (Fig. 1B top) for the immobilisation of the rats are fixed in such a way that the rats' brains are always positioned directly below the lower end of the gap between the ribs (Fig. 1B bottom).
Rats were fixed in Bouin solution (750 mL of saturated picric acid, 250 mL of formaldehyde 37%, and 50 mL of glacial acetic acid) or 4% paraformaldehyde (PFA) using vascular perfusion, and the samples were dissected and post-fixed by immersion (12 h).
Rats are fixed in a prone position during scanning.
Rats were fixed by perfusion with saline followed by 4% paraformaldehyde.
Rats were fixed in vivo by retrograde perfusion, as described in [ 7, 13].
The P25 rats were fixed with bars securing their head in the holder.
After adequate anesthesia, rats were fixed in the dorsal position on a surgical table.
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