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For the collection of free living stages, faeces from 8-day infected rats was collected and cultured at 19°C [ 26].
Blood from rats was collected daily.
After six weeks, the blood of all rats was collected prior to sacrifice and stored at -80°C until assayed.
Whole heparine-blood (1 ml) from donor rats was collected and platelets were stained with rhodamine 6G (Sigma Chemical, St . Louis USA) as described elsewhere [ 23].
Blood from LEW rats treated with TNFα in vivo as described above along with that from control rats was collected either from tail vein or via cardiac puncture.
The urine of the rats was collected at fixed times between day 1 and day 204, and the ICP was used to look for elements known to be present in the original fibers.
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E14 15 Sprague-Dawley rats were collected by cesarean section.
Tissues from male offspring rats were collected for pathological examination and microarray gene expression profiling.
OPCs derived from post-natal rats were collected by shake-off and differential adhesion after 8 14 days in mixed glial cell cultures (McCarthy and de Vellis, 1980).
OPCs derived from post-natal rats were collected by shake-off and differential adhesion after 8 14 days in mixed glial cell cultures12.
On day 43, blood samples from the rats were collected for fasting plasma glucose (FPG), total cholesterol, triglycerides, low-density lipoproteins (LDL-c), very low-density lipoprotein (VLDL-c) and high-density lipoprotein (HDL-c) assays through cardiac puncture under halothane anesthesia.
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