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RNA extracted from the mPFC collected from HI and LI rats was analyzed for efficiency of editing at each of the five editing sites (A, B, E, C, D) as well as the frequency of mRNA variants using high-throughput multiplexed transcript analysis (Morabito et al, 2010; Zhu et al, 2012); all 32 possible mRNA variants were detected in the rat mPFC (Supplementary Table 2).

Diaphragm muscle from streptozotocin-diabetic rats was analyzed with Affymetrix gene expression arrays.

Rotational behavior of these rats was analyzed, optical density of striatal tyrosine hydroxylase was calculated, and unbiased stereological counts of the substantia nigra were made.

Urine from control and infected rats was analyzed for the presence of cytokines with a multiplex antibody-immobilized bead immunoassay (Lincoplex KIT, Linco Research, Inc., St. Charles, MO).

PD i for hyperactivity, impulsiveness and inattention in the SHR and WKY rats was analyzed using nonparametric ranking by experimental session.

In the first experiment, only a single pooled urine sample from each experimental group (five rats) was analyzed for MEP, MPB, or triclosan.

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The biochemical parameters of the rats were analyzed with an autoanalyzer.

Outbred rats may more naturally reflect the varied genetics of the human population, but when small groups of outbred rats are analyzed, individual variation may be large.

Urine samples from rats in control and yuanhuapine treated rats were analyzed by ultra-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS).

Urinary samples from control and MGS treated rats were analyzed by ultra-performance liquid chromatography/mass spectrometry (UPLC MS) in positive ionization mode.

Data from experiments that tested the effect of the unilateral optogenetic inhibition of CeA CRF neurons on the escalation of alcohol self-administration in dependent rats were analyzed using two-way repeated-measures ANOVA, with time and laser illumination as within-subjects factors (see Figs. 4 and 5, Supplementary Figures 4, 5, 6).

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