Sentence examples for rats was added from inspiring English sources

An additional group of spontaneously breathing rats was added to discriminate the effect of surgical procedure and anesthesia in the brain.

Moreover, 11 extra rats were added to the vehicle group.

For suppression assays, 1×105 CD4+ T-cells or CD8+ T-cells from naïve or treated LEW rats were added to the standard MLR assay (using naïve LEW T-cells as responder cells) as described above.

50 μl of sera from treated or untreated rats were added to CTLL-2 cells cultured at 2 × 105 cells/ml on 96-well plates at 37°C in assay medium not containing IL-2.

An additional group of spontaneously breathing rats (Spont) was added to discriminate the effect of the surgical procedure and anesthesia on brain activation.

On the day of adult eclosion, food in the form of rat chow was added to the cup and maintained at ad libitum levels.

Following washes with staining buffer, secondary antibody, anti-rat FITC was added for 30 mins on ice.

Because spider silk is prone to strong auto-fluorescence signals, Alexa 488 goat anti-rat (Invitrogen) was added to the samples to mark the spider silk fibres by unspecific attachment.

In other experiments, at the time of challenge, naïve rat serum was added as an opsonin, or 1 mM N-methyl-L-arginine acetate salt (Sigma Aldrich, St Louis, MO) as a nitric oxide inhibitor [43].

After washing, the detection antibody (goat anti-rat VEGF) was added and incubated for a further 2 h at room temperature.

Cells were then pelleted by centrifuging at 500 × g for 5 min. All supernatant was removed from the cell pellet except for approximately 200 μl. 10 μg of rat IgG (BD biosciences; San Jose, CA) was then added and each sample incubated on ice for 15 min. Rat IgG was added to the samples to block nonspecific antibody binding to the cells.