After washing with Wash Buffer (Dako), the primary antibodies were detected with LSAB2 System-HRP for use on rat specimens (Dako) according to the manufacturer's instructions.
CDCs, derived from rat specimens, readily formed clones when plated as single cells (Figure 6).
Additionally, electron micrographs of human and rat specimens at various time points after radiation support our findings by revealing a similar pattern of myelin sheath degradation over time post radiation with absence of significant axonal damage.
Cytb, IRBP and COI sequences were generated for 122, 120 and 116 rat specimens respectively.
As the rat Osteocalcin concentrations was proportional to the OD value, the osteocalcin concentration in rat specimens could be obtained by drawing standard curve.
To these aims, we first sequenced two mitochondrial and one nuclear genes from rat specimens coming from Southeast Asia (Thailand, Cambodia and Lao People's Democratic Republic) to perform phylogenetic reconstructions.
Considering their preponderant place in epidemiological surveys, 4 worldwide black rat specimens (identified in [ 36]) were added to the sample set.
Because of the limited availability of rat specimens that are positive for HEV RNA, we first transmitted the rat HEV to a laboratory rat (Wistar) to produce a large amount of virus for RNA extraction and genome amplification.
Isolates originating from multimammate rat specimens obtained in a particular location tended to cluster, and conversely sequences present in specific branches of the trees in many cases originated from a single location or few locations not far from each other.
The rat sample sections were stained with anti-rabbit, anti-mouse or avidin-biotinylated HRP-conjugate antibodies according to the staining protocol (LSAB 2 System-HRP for use on rat specimens #K0609; Dako Cytomation, Tokyo, Japan), and then reacted with 3,30-diaminobenzidine.
