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The expression pattern of HSPs is not only tissue-specific [ 34, 120 ] but also species-specific, as revealed by decrease, moderately increase, and overexpression of HSP73 in COS-7 cells (African green monkey kidney cells), A549 cells (human lung tumor cells), and rats kidney cells, in exposure to 100, 200, or 400 M NiCl2, respectively, for 4 days [ 121 ].
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The researchers, from the University of Heidelberg in Germany and other European institutions, observed what they called tunneling nanotubes among embryonic human kidney cells and normal rat kidney cells.
Multipotent renal progenitor cells (MRPCs) isolated by culturing rat kidney cells showed high self-renewal capacity and expression of genes involved in kidney development, like Pax2 [113].
In this report, we examined the effect of 3D surfaces on the activation states of Rho GTPases within NIH 3T3 fibroblasts and normal rat kidney cells.
Moreover, a recent publication indicates that disruption of cell cycle in normal rat kidney cells (NRK) is important for VSV ability to kill cells [36].
As negative control, we used KNRK cells (Kirsten murine sarcoma virus transformed Normal Rat Kidney cells) expressing the substance-P receptor.
We therefore concluded that, contradictory to what has been seen in primary T-lymphocytes and normal rat kidney cells, cell proliferation is not a key player in supporting VSV replication in HCC.
NIH/3T3, Rat kidney cells (RK3) were purchased from ATCC and grown in Dulbelcco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 1% l-glutamine (Gibco-BRL).
We next tested if overexpressed DHFR due to the miR-24-TS-SNP can also induce anchorage independent growth in two additional rodent cell lines (NIH3T3 cells and RK3-rat kidney cells) and human breast epithelial MCF10A cells, in addition to CHO cells.
Next, the dynamic localization pattern of PC2 in rat kidney cells was investigated.
A single dose of 4 mmol/kg of TCE increased the frequency of micronucleated rat kidney cells [ 17].
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