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Non-immunized rats or rats immunized with x-irradiated T9 cells (n = 8) demonstrated a lack of immunity by the presence of growing T9 tumors.
The principal expression of immunity elicited in syngeneic rats immunized with rat hepatoma membrane fractions was the development of a tumour specific antibody response.
Similarly, grafts of fetal hippocampus to rats immunized with nerve growth factor were not significantly different from grafts to host rats immunized with cytochrome c.
Lymph node cells from E3 rats immunized with the 22a1-7E TCR protein were prepared 10 days after immunization and were fused with myeloma cells (P3X63-Ag8.653) as described previously [ 14].
It was shown that rats immunized with the immunogens generated antibodies specific for GnRH.
Antibodies anti-AP2-exp were obtained from rats immunized with the recombinant GST-tagged N-terminal region of the AP2 protein using standard protocols by Genscript.
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Reynolds et al [36] recently using an experimental autoimmune glomerulonephritis (EAG) model have shown that WKY rat immunized with the 15mer pcol (24 38) - (FTRHSQTTANPSCPE), a stretch of a human B cell epitope in the N terminal of the alpha 3 chain of type IV collagen; elicited either strong B cell or T cell response.
In contrast, antibodies from a control rat immunized with PBS had no effect on patient IgE binding to the allergen (Additional file 1: Figure S2).
AIL rats were immunized with 20 µg of MOG per rat while congenic rats were immunized with sex-specific MOG doses, 15 and 22 µg/rat in females (depending on MOG batch) and 30 µg/rat in males.
Lew1AV1 rats were immunized with Cit-rat serum albumin (RSA) or unmodified RSA, and antibody and T-cell responses were evaluated.
Six-week-old female WKY, LEW and LEW.W Crgn1,2 rats were immunized with 0.5 mg/kg recombinant rat α3(in)Ncompletemplete Freund's adjuvant by intramuscular injection.
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